Coding

Part:BBa_M11061:Design

Designed by: Dan Nelson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-13)


pfaD omega-3 polyunsaturated fatty acid synthase (mutated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1024
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1396
    Illegal AgeI site found at 1614
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations were made to an earlier entry of the same gene to exclude 2 Pst1 sites (747,798).


Source

Photobacterium profundum SS9 chromosome 1: NC_006370 (JGI)

References